Journal article
Plant-derived virus-like particle vaccines drive cross-presentation of influenza A hemagglutinin peptides by human monocyte-derived macrophages
AI Makarkov, M Golizeh, E Ruiz-Lancheros, AA Gopal, IN Costas-Cancelas, S Chierzi, S Pillet, N Charland, N Landry, I Rouiller, PW Wiseman, M Ndao, BJ Ward
Npj Vaccines | SPRINGERNATURE | Published : 2019
Abstract
A growing body of evidence supports the importance of T cell responses to protect against severe influenza, promote viral clearance, and ensure long-term immunity. Plant-derived virus-like particle (VLP) vaccines bearing influenza hemagglutinin (HA) have been shown to elicit strong humoral and CD4+ T cell responses in both pre-clinical and clinical studies. To better understand the immunogenicity of these vaccines, we tracked the intracellular fate of a model HA (A/California/07/2009 H1N1) in human monocyte-derived macrophages (MDMs) following delivery either as VLPs (H1-VLP) or in soluble form. Compared to exposure to soluble HA, pulsing with VLPs resulted in ~3-fold greater intracellular a..
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Awarded by European Commission
Funding Acknowledgements
This work was supported by the Canadian Institutes of Health Research (Grant # UI301202) and the Ministere de l'Economie et de l'Innovation (MEI) of Quebec with project oversight by Genome Quebec. P. W. W. acknowledges support from a Natural Sciences and Engineering Research Council of Canada (NSERC) discovery grant (Grant # RGPIN-2017-05005). The authors thank Dr. John Presley at McGill University for helpful discussions during the planning of experiments and manuscript preparation. The authors thank the Molecular Imaging Platform of Research Institute of the McGill University Health Centre and its staff for the kind services provided.