Journal article

Hordein Accumulation in Developing Barley Grains

Gregory J Tanner, Michelle L Colgrave, Malcolm J Blundell, Crispin A Howitt, Antony Bacic

FRONTIERS IN PLANT SCIENCE | FRONTIERS MEDIA SA | Published : 2019

Abstract

The temporal pattern of accumulation of hordein storage proteins in developing barley grains was studied by enzyme-linked immunosorbent assay (ELISA), western blot and liquid chromatography tandem mass spectrometry (LC-MS/MS). Hordein accumulation was compared to the pattern seen for two abundant control proteins, serpin Z4 (an early accumulator) and lipid transferase protein (LTP1, a late accumulator). Hordeins were detected from 6 days post-anthesis (DPA) and peaked at 30 DPA. Changes in fresh weight indicate that desiccation begins at 20 DPA and by 37 DPA fresh weight had decreased by 35%. ELISA analysis of hordein content, expressed on a protein basis, increased to a maximum at 30 DPA fo..

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