Detection of humoral immunity to mycobacteria causing leprosy in Eurasian red squirrels (Sciurus vulgaris) using a quantitative rapid test
Anna-Katarina Schilling, Anouk van Hooij, Paul Corstjens, Peter WW Lurz, Jorge DelPozo, Karen Stevenson, Anna Meredith, Annemieke Geluk
European Journal of Wildlife Research | Springer Verlag | Published : 2019
Eurasian red squirrels (Sciurus vulgaris, ERS) in the British Isles are a recently discovered natural host for Mycobacterium leprae and Mycobacterium lepromatosis. Infected squirrels can develop skin lesions or carry the bacteria without showing clinical signs. Until now the clinical diagnosis of leprosy could only be confirmed in squirrels by isolating DNA of leprosy bacilli from carcasses or by establishing the presence of acid-fast bacilli in skin sections of carcasses with clinical signs. In this study, we assessed the performance of a field-friendly diagnostic test for detection of M. leprae/M. lepromatosis infection in ERS. This up-converting phosphor lateral flow assay (UCP-LFA) is we..View full abstract
This study was supported by the Q.M. GastmannWichers Foundation (to AG), the Principal's Career Development PhD Scholarship by the University of Edinburgh (to AKS), and the Scottish Government Rural and Environment Science and Analytical Services Division.