Journal article
RAB6 and microtubules restrict protein secretion to focal adhesions
L Fourriere, A Kasri, N Gareil, S Bardin, H Bousquet, D Pereira, F Perez, B Goud, G Boncompain, S Miserey-Lenkei
Journal of Cell Biology | ROCKEFELLER UNIV PRESS | Published : 2019
Open access
Abstract
To ensure their homeostasis and sustain differentiated functions, cells continuously transport diverse cargos to various cell compartments and in particular to the cell surface. Secreted proteins are transported along intracellular routes from the endoplasmic reticulum through the Golgi complex before reaching the plasma membrane along microtubule tracks. Using a synchronized secretion assay, we report here that exocytosis does not occur randomly at the cell surface but on localized hotspots juxtaposed to focal adhesions. Although microtubules are involved, the RAB6-dependent machinery plays an essential role. We observed that, irrespective of the transported cargos, most post-Golgi carriers..
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Awarded by Seventh Framework Programme
Funding Acknowledgements
Work performed in the F. Perez laboratory was funded by Centre National de la Recherche Scientifique, the Fondation pour la Recherche Medicale (FRM DEQ20120323723 and FRM FDT20150532154 to L. Fourriere), the Labex CellTisPhyBio (L. Fourriere), and the Agence Nationale de la Recherche (ANR-12-BSV2-0003-01). Work performed in the B. Goud laboratory was supported by a European Research Council advanced grant (project 339847 MYODYN), Agence Nationale de la Recherche (to A. Kasri), ARC (to A. Kasri), and Institut Curie (to A. Kasri). The Goud and Perez teams are members of Labex CelTisPhyBio (11-LBX-0038) and Idex Paris Sciences et Lettres (ANR-10-IDEX-0001-02 PSL).