Journal article

Development of an Ultrasensitive Impedimetric Immunosensor Platform for Detection of Plasmodium Lactate Dehydrogenase

Yu Kong Low, Jianxiong Chan, Gita V Soraya, Christelle Buffet, Chathurika D Abeyrathne, Duc H Huynh, Efstratios Skafidas, Patrick Kwan, Stephen J Rogerson

SENSORS | MDPI | Published : 2019

Abstract

Elimination of malaria is a global health priority. Detecting an asymptomatic carrier of Plasmodium parasites to receive treatment is an important step in achieving this goal. Current available tools for detection of malaria parasites are either expensive, lacking in sensitivity for asymptomatic carriers, or low in throughput. We investigated the sensitivity of an impedimetric biosensor targeting the malaria biomarker Plasmodium lactate dehydrogenase (pLDH). Following optimization of the detection protocol, sensor performance was tested using phosphate-buffered saline (PBS), and then saliva samples spiked with pLDH at various concentrations. The presence of pLDH was determined by analyzing t..

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Grants

Awarded by Bill & Melinda Gates Foundation


Awarded by National Health and Medical Research Council of Australia


Awarded by Medical Research Future Fund Practitioner Fellow


Funding Acknowledgements

This research was funded by Bill & Melinda Gates Foundation's Grand Challenges Explorations, grant number OPP1151367. S.J.R. is supported by a Program Grant from the National Health and Medical Research Council of Australia APP1092789. P.K. is a Medical Research Future Fund Practitioner Fellow (1136427).