Bio-engineering a tissue flap utilizing a porous scaffold incorporating a human induced pluripotent stem cell-derived endothelial cell capillary network connected to a vascular pedicle
Anne M Kong, Kiryu K Yap, Shiang Y Lim, Diego Marre, Alice Pebay, Yi-wen Gerrand, Jarmon G Lees, Jason A Palmer, Wayne A Morrison, Geraldine M Mitchell
Acta Biomaterialia | ELSEVIER SCI LTD | Published : 2019
Tissue flaps are used to cover large/poorly healing wounds, but involve complex surgery and donor site morbidity. In this study a tissue flap is assembled using the mammalian body as a bioreactor to functionally connect an artery and vein to a human capillary network assembled from induced pluripotent stem cell-derived endothelial cells (hiPSC ECs). In vitro: Porous NovoSorb™ scaffolds (3 mm × 1.35 mm) were seeded with 200,000 hiPSC ECs ± 100,000 human vascular smooth muscle cells (hvSMC), and cultured for 1-3 days, with capillaries formed by 24 h which were CD31+, VE-Cadherin+, EphB4+, VEGFR2+ and Ki67+, whilst hvSMCs (calponin+) attached abluminally. In vivo: In SCID mice, bi-lateral epiga..View full abstract
Awarded by Australian Research Council Future Fellowship
Awarded by NHMRC Senior Research Fellowship
The authors acknowledge the assistance of the Experimental Medical and Surgical Unit (Sue Mc Kay, Anna Deftereos, Liliana Pepe, and Amanda Rixon) at St Vincent's Hospital Melbourne.This research was funded by grants from the Research Endowment Fund, St Vincent's Hospital, Melbourne, Australia; the Australian Catholic University; the Stafford Fox Foundation, Australia; and the Victorian State Government's Department of Innovation, Industry and Regional Development's Operational Infrastructure Support Program.KKY is supported by scholarships from the National Health & Medical Research Council; Australia and New Zealand Hepatic, Pancreatic, and Biliary Association; and St Vincent's Institute Foundation. AP is supported by an Australian Research Council Future Fellowship (FT140100047) and an NHMRC Senior Research Fellowship (1154389).