Journal article

The potential role of human multidrug resistance protein 1 (MDR1) and multidrug resistance-associated protein 2 (MRP2) in the transport of Huperzine A in vitro

Ziyan Fei, Mengyun Hu, Larry Baum, Patrick Kwan, Tao Hong, Chunbo Zhang

XENOBIOTICA | TAYLOR & FRANCIS LTD | Published : 2020

Abstract

1. More than 30% of epilepsy patients suffer pharmacoresistance. Transport of antileptic drugs by P-glycoprotein (P-gp) and MRP2 plays an important role in drug-resistant epilepsy. Huperzine A (Hup-A) is a natural compound, which might have potential in treating neurological disorders including epilepsy and Alzheimer's disease. In this study, we investigated whether human P-gp and MRP2 transport Hup-A.2. LLC-PK1 and MDCKII cells transfected with human P-gp or MRP2 were used to establish concentration equilibrium transport assays (CETAs) and determine the transport profile of Hup-A. The expression of P-gp and MRP2 was detected by qPCR and western blotting. The transport function of P-gp and M..

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Grants

Awarded by National Natural Science Foundation of China


Awarded by Natural Science Foundation of Jiangxi Province


Awarded by Science and Technology Project of Jiangxi Provincial Education Department


Awarded by Science and Technology Project of Health and Family Planning Commission of Jiangxi Provence


Funding Acknowledgements

Financial support was provided by National Natural Science Foundation of China under Grants [NSFC-31771400, NSFC-81728006, NSFC-81501129, NSFC-81860662]; Natural Science Foundation of Jiangxi Province under Grants [20161BAB215200, 20171ACB21001, 20171BCB23029]; Science and Technology Project of Jiangxi Provincial Education Department under Grant [150111]; and Science and Technology Project of Health and Family Planning Commission of Jiangxi Provence under Grant [20175534].