Journal article

Methods for Assessing Scaffold Vascularization In Vivo

Jiang-Hui Wang, Jinying Chen, Shyh-Ming Kuo, Geraldine M Mitchell, Shiang Y Lim, Guei-Sheung Liu, S BottcherHaberzeth (ed.), T Biedermann (ed.)

Methods in molecular biology (Clifton, N.J.) | HUMANA PRESS INC | Published : 2019


The success of tissue engineering hinges on the rapid and sufficient vascularization of the neotissue. For efficient vascular network formation within three-dimensional (3D) constructs, biomaterial scaffolds that can support survival of endothelial cells as well as formation and maturation of a capillary network in vivo are highly sought after. Here, we outline a method to biofabricate 3D porous collagen scaffolds that can support extrinsic and intrinsic vascularization using two different in vivo animal models-the mouse subcutaneous implant model (extrinsic vascularization, capillary growth within the scaffold originating from host tissues outside the scaffold) and the rat tissue engineerin..

View full abstract