Journal article

Evaluation of Total Donor Endothelial Viability After Endothelium-Inward Versus Endothelium-Outward Loading and Insertion in Descemet Membrane Endothelial Keratoplasty

Elaine W Chong, Francisco Bandeira, Prema Finn, Jodhbir S Mehta, Elsie Chan

Cornea | LIPPINCOTT WILLIAMS & WILKINS | Published : 2020

Abstract

PURPOSE: This study compares endothelial cell density (ECD) and viability between 2 different techniques used to prepare and insert Descemet membrane endothelial keratoplasty (DMEK) donor tissues. The first technique uses the naturally forming Descemet membrane (DM) scroll where the endothelial cells face outward; in the second technique, the DM is folded into thirds (trifold) with the endothelial cells facing inward. METHODS: Eighteen cadaveric corneas from 9 donors (matched pairs) were used to compare the 2 tissue-insertion techniques. In the scroll, endothelium-outward technique, standard DMEK preparation was used, and the naturally forming DM scroll was inserted into a Geuder Cartridge. ..

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