Experimental Cerebral Malaria Spreads along the Rostral Migratory Stream
Angelika Hoffmann, Johannes Pfeil, Julieta Alfonso, Felix T Kurz, Felix Sahm, Sabine Heiland, Hannah Monyer, Martin Bendszus, Ann-Kristin Mueller, Xavier Helluy, Mirko Pham
PLOS PATHOGENS | PUBLIC LIBRARY SCIENCE | Published : 2016
It is poorly understood how progressive brain swelling in experimental cerebral malaria (ECM) evolves in space and over time, and whether mechanisms of inflammation or microvascular sequestration/obstruction dominate the underlying pathophysiology. We therefore monitored in the Plasmodium berghei ANKA-C57BL/6 murine ECM model, disease manifestation and progression clinically, assessed by the Rapid-Murine-Coma-and-Behavioral-Scale (RMCBS), and by high-resolution in vivo MRI, including sensitive assessment of early blood-brain-barrier-disruption (BBBD), brain edema and microvascular pathology. For histological correlation HE and immunohistochemical staining for microglia and neuroblasts were o..View full abstract
Awarded by German Centre for Infection Research (Deutsches Zentrum fuer Infektionsforschung, DZIF)
This work was supported by an award from the German Society of Pediatric Infectious Diseases (Deutsche Gesellschaft fuer Paediatrische Infektiologie, DGPI) to JP. AH, FTK and FS receive funding from a postdoctoral stipend of the Medical Faculty of the University of Heidelberg. JP received funding from the German Centre for Infection Research (Deutsches Zentrum fuer Infektionsforschung, DZIF) (grant no. 80006013). MP is supported by a memorial stipend from the Else-Kroner-Fresenius-Foundation. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.