Journal article

Nucleosome remodeling at the IL-12 p40 promoter is a TLR-dependent, Rel-independent event

AS Weinmann, DM Mitchell, S Sanjabi, MN Bradley, A Hoffmann, HC Liou, ST Smale

NATURE IMMUNOLOGY | NATURE AMERICA INC | Published : 2001

Abstract

Lipopolysaccharide (LPS) induction of the gene encoding interleukin 12 p40 requires remodeling of a promoter-encompassing nucleosome and the Toll-like receptor (TLR)-mediated activation of a c-Rel-containing complex. Analysis of TLR4-mutant mice revealed that remodeling requires TLR signaling. However, Rel proteins and other proteins required for transcription of an integrated p40 promoter were insufficient for remodeling. c-Rel was also unnecessary for remodeling, as remodeling was observed in c-Rel-/- macrophages, which lack p40 transcripts. These results suggest that remodeling requires TLR signaling pathways that diverge from the c-Rel activation pathways. The factors that stimulate remo..

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University of Melbourne Researchers