Journal article
Wheat wounding-responsive HD-Zip IV transcription factor GL7 is predominantly expressed in grain and activates genes encoding defensins
N Kovalchuk, W Wu, N Bazanova, N Reid, R Singh, N Shirley, O Eini, AAT Johnson, P Langridge, M Hrmova, S Lopato
Plant Molecular Biology | SPRINGER | Published : 2019
Abstract
Key message: Several classes of transcription factors are involved in the activation of defensins. A new type of the transcription factor responsible for the regulation of wheat grain specific defensins was characterised in this work. Abstract: HD-Zip class IV transcription factors constitute a family of multidomain proteins. A full-length cDNA of HD-Zip IV, designated TaGL7 was isolated from the developing grain of bread wheat, using a specific DNA sequence as bait in the Y1H screen. 3D models of TaGL7 HD complexed with DNA cis-elements rationalised differences that underlined accommodations of binding and non-binding DNA, while the START-like domain model predicted binding of lipidic molec..
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Awarded by Australian Research Council
Funding Acknowledgements
We acknowledge the contributions of Ainur Ismagul and Serik Eliby in plant transformation. We thank Margaret Pallotta for technical assistance with BAC library screening and promoter cloning. Ursula Langridge, Lorraine Carruthers and Alex Kovalchuk are thanked for their assistance with growing of plants in the glasshouse. This work was supported by the Australian Research Council (Grant No. LP120100201 to M.H. and S.L.), the Grains Research and Development Corporation and the Government of South Australia.