Journal article
Protein-eye view of the in meso crystallization mechanism
L Van 't Hag, L De Campo, N Tran, A Sokolova, R Trenker, ME Call, MJ Call, CJ Garvey, AE Leung, TA Darwish, A Krause-Heuer, R Knott, TG Meikle, CJ Drummond, R Mezzenga, CE Conn
Langmuir | AMER CHEMICAL SOC | Published : 2019
Abstract
Copyright © 2019 American Chemical Society. For evolving biological and biomedical applications of hybrid protein-lipid materials, understanding the behavior of the protein within the lipid mesophase is crucial. After more than two decades since the invention of the in meso crystallization method, a protein-eye view of its mechanism is still lacking. Numerous structural studies have suggested that integral membrane proteins preferentially partition at localized flat points on the bilayer surface of the cubic phase with crystal growth occurring from a local fluid lamellar Lα phase conduit. However, studies to date have, by necessity, focused on structural transitions occurring in the lipid me..
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Awarded by Appalachian Regional Commission
Funding Acknowledgements
We acknowledge the Australian Center for Neutron Scattering (ACNS), Australian Nuclear Science and Technology Organization (ANSTO) for providing neutron research facilities, and the Australian National Deuteration Facility (supported by the National Collaborative Research Infrastructure Strategy (NCRIS), an initiative of the Australian Government) for providing chemical deuteration facilities. We also acknowledge the use of the SAXS/WAXS beamline at the Australian Synchrotron and thank Drs. Nigel M. Kirby, Adrian M. Hawley, and Stephen T. Mudie for assistance, as well as Drs. Pimm Vongsvivut and Mark Tobin for use of their microscope. We also thank Dr. Janet Newman for help with robotic set-up of plates at the C<SUP>3</SUP> Collaborative Crystallization Center, CSIRO, Parkville, Australia. We acknowledge AINSE, Ltd. for providing a Post-Graduate Research Award (PGRA) to LvH enabling work at the ACNS. N.T. is supported by the RMIT Vice Chancellor's Research Fellowship. C.E.C. is the recipient of an ARC DECRA Fellowship: DE160101281 and an RMIT Vice Chancellor's Senior Research Fellowship. The GpA and DAP12 crystallography was enabled by an ARC Future Fellowship (FT120100145) to M.J.C., an ARC QEII Fellowship (DP110104369) to M.E.C. and NHMRC grants GNT1011352 and GNT1030902.