PRODUCTION OF IPSCS FROM A SMALL VOLUME OF CRYOPRESERVED HUMAN UMBILICAL CORD BLOOD BUFFY COAT UNDER "GMP-COMPLIANT" CONDITIONS

Abstract

Background & Aim An iPSC bank derived from cord blood selected on the basis of human leukocyte antigens (HLA) requires the development and validation of protocols to generate iPSC lines under good manufacturing practice (GMP) conditions. Working towards this goal, we aim to create clinically compliant GMP grade iPSCs from small volumes of banked cord blood (CB) buffy coat samples for potential clinical use. Methods, Results & Conclusion We have established a protocol to generate iPSC lines from a minimum of 50 µl of cryopreserved CB buffy coat using defined medium and reagents manufactured under GMP. This allows us to use only segments or pilot tube of the stored CB unit without compromising..