Development and validation of a multiplexed-tandem qPCR tool for diagnostics of human soil-transmitted helminth infections
Katharina Stracke, Naomi Clarke, Camille V Awburn, Susana Vaz Nery, Virak Khieu, Rebecca J Traub, Aaron R Jex
PLOS Neglected Tropical Diseases | Public Library of Science | Published : 2019
Soil-transmitted helminths (STH) are a major cause of morbidity in tropical developing countries with a global infection prevalence of more than one billion people and disease burden of around 3.4 million disability adjusted life years. Infection prevalence directly correlates to inadequate sanitation, impoverished conditions and limited access to public health systems. Underestimation of infection prevalence using traditional microscopy-based diagnostic techniques is common, specifically in populations with access to benzimidazole mass treatment programs and a predominance of low intensity infections. In this study, we developed a multiplexed-tandem qPCR (MT-PCR) tool to identify and quanti..View full abstract
Awarded by NHMRC Career Development Fellowship
This work, including the efforts of ARJ, was funded by a NHMRC Career Development Fellowship (APP1126395). KS, CVA, RJT and ARJ are supported by the Victorian State Government Operational Infrastructure Support and Australian Government National Health and Medical Research Council Independent Research Institute Infrastructure Support Scheme. KS is supported by a Melbourne Research Scholarship (Australian Government) and the Riady Scholarship (Victoria, Australia). The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.