Journal article

Geometric characteristics of collagen have independent prognostic significance in breast ductal carcinoma in situ: an image analysis study

Michael S Toss, Islam M Miligy, Kylie L Gorringe, Abdulbaqi AlKawaz, Karuna Mittal, Ritu Aneja, Ian O Ellis, Andrew R Green, Ioannis Roxanis, Emad A Rakha



Collagen plays a key role in normal and malignant tissue homeostasis. While the prognostic significance of collagen fiber remodeling in invasive breast cancer has been studied, its role in ductal carcinoma in situ (DCIS) remains poorly defined. Using image analysis, we aimed to evaluate the prognostic significance of the geometric characteristics of collagen surrounding DCIS. A large well-characterized cohort of DCIS comprising pure DCIS (n = 610) and DCIS coexisting with invasive carcinoma (n = 180) were histochemically stained for collagen using picrosirius red. ImageJ software was used to assess collagen density, degree of collagen fiber dispersion and directionality in relation to DCIS d..

View full abstract


Funding Acknowledgements

This research was supported and funded by the Egyptian Ministry of Higher Education and Scientific Research. KLG was supported by a Yamagiwa-Yoshida Memorial international study grant from the Union for International Cancer Control. We thank the Nottingham Health Science Biobank and Breast Cancer Now Tissue Bank for the provision of tissue samples.