Single-Cell Transcriptomics Identifies the Adaptation of Scart1( )V gamma 6( ) T Cells to Skin Residency as Activated Effector Cells
Likai Tan, Inga Sandrock, Ivan Odak, Yuval Aizenbud, Anneke Wilharm, Joana Barros-Martins, Yaara Tabib, Alina Borchers, Tiago Amado, Lahiru Gangoda, Marco J Herold, Marc Schmidt-Supprian, Jan Kisielow, Bruno Silva-Santos, Christian Koenecke, Avi-Hai Hovav, Christian Krebs, Immo Prinz, Sarina Ravens
CELL REPORTS | CELL PRESS | Published : 2019
IL-17-producing γδ T cells express oligoclonal Vγ4+ and Vγ6+ TCRs, mainly develop in the prenatal thymus, and later persist as long-lived self-renewing cells in all kinds of tissues. However, their exchange between tissues and the mechanisms of their tissue-specific adaptation remain poorly understood. Here, single-cell RNA-seq profiling identifies IL-17-producing Vγ6+ T cells as a highly homogeneous Scart1+ population in contrast to their Scart2+ IL-17-producing Vγ4+ T cell counterparts. Parabiosis demonstrates that Vγ6+ T cells are fairly tissue resident in the thymus, peripheral lymph nodes, and skin. There, Scart1+ Vγ6+ T cells display tissue-specific gene expression signatures in the sk..View full abstract
Awarded by Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany's Excellence Strategy
The work supported by Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany's Excellence Strategy - EXC 2155 "RESIST'' - Project ID 39087428 to S.R. and I.P.; SFB900 - Project ID 158989968 to S.R., C. Koenecke and I.P; SFB1192/A5 to C. Krebs and RA 3077/1-1 to S.R.; and the state of Lower Saxony COALITION (to S.R. and I.P.). L.T., I.O., J.B.-M., and A.W. were supported by the Hannover Biomedical Research School. We would like to acknowledge the assistance of the Cell Sorting Core Facility of the Hannover Medical School, and we thank Matthias Ballmaier for advice and Abdi Demera for animal care taking.