Journal article
The impact of sample storage on molecular-based detection of Mycoplasma genitalium
GL Murray, JP Su, J Birnie, J Danielewski, DA Machalek, CS Bradshaw, TRH Read, AM Costa, SM Garland
Journal of Applied Microbiology | OXFORD UNIV PRESS | Published : 2019
DOI: 10.1111/jam.14359
Abstract
Aims: Mycoplasma genitalium causes a common, sexually transmitted bacterial infection. This study assessed the detection of M. genitalium in stored urine samples to understand the impact of sample storage on M. genitalium detection. Methods: Aliquots of M. genitalium-positive urine (n = 20 patients) were stored at either room temperature (22°C) or 4°C, without a preservative. At weekly intervals, samples were tested using the commercial test ResistancePlus MG® (SpeeDx®, Australia). We report the analysis at 1 week, an acceptable collection-to-test turnaround time, with further analysis over 5 weeks to illustrate degradation trends. Results: After storing at 4°C, the proportion of specimens t..
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Awarded by National Health and Medical Research Council
Funding Acknowledgements
We acknowledge the support of SpeeDx in providing the detection kits for the assays. This work was supported by an Innovations Connections grant from the Department of Industry, Innovation and Science, The Australian Federal Government (grant number ICG000220). T.R.H.R. was supported by early career fellowship number 1091536 from the National Health and Medical Research Council.