Journal article

Clove Oil and AQUI-S Efficacy for Zebrafish Embryo, Larva, and Adult Anesthesia

Ophelia Ehrlich, Anthony Karamalakis, Aaron James Krylov, Stefanie Dudczig, Kathryn Louise Hassell, Patricia Regina Jusuf



Since the use of the zebrafish Danio rerio genetic model organism within the scientific research community continues to grow rapidly, continued procedural refinement to support high-quality, reproducible research and improve animal welfare remains an important focus. As such, anesthesia remains one of the most frequent procedures conducted. Here, we compared the effectiveness of clove oil (active ingredient eugenol) and AQUI-S (active ingredient iso-eugenol) with the currently most commonly used tricaine/MS-222 (ethyl 3-aminobenzoate methanesulfonate) and benzocaine anesthesia. We focused on embryos (1 day postfertilization), larvae (5 days postfertilization), and adults (9-11 months) and fo..

View full abstract

University of Melbourne Researchers


Funding Acknowledgements

The authors thank the University of Melbourne Faculty of Science animal ethics committee and especially animal welfare officer Shari Cohen for continued discussion and advice on their study. They acknowledge use of the Walter and Eliza Hall Institute of Medical Research zebrafish facility. This project was supported by a Tecniplast research scholarship to A. K.