Deleting Suppressor of Cytokine Signaling-3 in chondrocytes reduces bone growth by disrupting mitogen-activated protein kinase signaling
X Liu, AA D'Cruz, J Hansen, BA Croker, KE Lawlor, NA Sims, IP Wicks
Osteoarthritis and Cartilage | ELSEVIER SCI LTD | Published : 2019
OBJECTIVE: To investigate the impact of deleting Suppressor of Cytokine Signaling (SOCS)-3 (SOCS3) in chondrocytes during murine skeletal development. METHOD: Mice with a conditional Socs3 allele (Socs3fl/fl) were crossed with a transgenic mouse expressing Cre recombinase under the control of the type II collagen promoter (Col2a1) to generate Socs3Δ/Δcol2 mice. Skeletal growth was analyzed over the lifespan of Socs3Δ/Δcol2 mice and controls by detailed histomorphology. Bone size and cortical bone development was evaluated by micro-computed tomography (micro-CT). Growth plate (GP) zone width, chondrocyte proliferation and apoptosis were assessed by immunofluorescence staining for Ki67 and TUN..View full abstract
Awarded by National Health and Medical Research Council of Australia
Supported by the Reid Charitable Trusts, the Arthritis Foundation of Australia, the National Health and Medical Research Council of Australia (grants 1023407 and 1016647 to Dr. Wicks, and grant 1120978 to Dr. Sims) and the Victorian State Government (Operational Infrastructure Grant). I.P. W's lab has received funding from CSL and MedImmune for research on cytokine antagonists.