Journal article

Calpain 2 and PTP1B function in a novel pathway with Src to regulate invadopodia dynamics and breast cancer cell invasion

Christa L Cortesio, Keefe T Chan, Benjamin J Perrin, Nicholas O Burton, Sheng Zhang, Zhong-Yin Zhang, Anna Huttenlocher

JOURNAL OF CELL BIOLOGY | ROCKEFELLER UNIV PRESS | Published : 2008

Abstract

Invasive cancer cells form dynamic adhesive structures associated with matrix degradation called invadopodia. Calpain 2 is a calcium-dependent intracellular protease that regulates adhesion turnover and disassembly through the targeting of specific substrates such as talin. Here, we describe a novel function for calpain 2 in the formation of invadopodia and in the invasive abilities of breast cancer cells through the modulation of endogenous c-Src activity. Calpain-deficient breast cancer cells show impaired invadopodia formation that is rescued by expression of a truncated fragment of protein tyrosine phosphatase 1B (PTP1B) corresponding to the calpain proteolytic fragment, which indicates ..

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