The Effects of Aging on Corneal and Ocular Surface Homeostasis in Mice
Manikkuwadura Eranda Harshan De Silva, Lisa J Hill, Laura E Downie, Holly R Chinnery
Investigative Ophthalmology & Visual Science | ASSOC RESEARCH VISION OPHTHALMOLOGY INC | Published : 2019
Purpose: Aging is a risk factor for dry eye disease. The aim of this study was to investigate if aging is associated with a range of signs of dry eye disease, including tear hyperosmolarity, reduced nerve density, and increased dendritic cell number, in mice. Method: Healthy C57BL/6 female mice, aged 2 months (young, n = 10) and 22 months (aged, n = 11) were used. Clinical assessments included corneal sensitivity (Cochet-Bonnet esthesiometry), tear secretion (Phenol red thread test), tear film osmolarity (TearLab osmometer), and corneal thickness (optical coherence tomography). The sum length of the corneal superficial terminals and sub-basal nerves, density of vertical nerve projections, an..View full abstract
Awarded by National Health and Medical Research Council
The authors thank the Advanced Microscopy Facility at The Florey for support. We also extend our gratitude to Monique Taylor (University of Melbourne, Australia) who volunteered to mask the dendritic cell and nerve files.Supported by a National Health and Medical Research Council Project Grant (APP1126540; HRC).