Journal article

PD-L1 expression on nonclassical monocytes reveals their origin and immunoregulatory function

Mariaelvy Bianchini, Johan Duchene, Donato Santovito, Maximilian J Schloss, Maximilien Evrard, Holger Winkels, Maria Aslani, Sarajo K Mohanta, Michael Horckmans, Xavier Blanchet, Michael Lacy, Philipp von Hundelshausen, Dorothee Atzler, Andreas Habenicht, Norbert Gerdes, Jaroslav Pelisek, Lai Guan Ng, Sabine Steffens, Christian Weber, Remco TA Megens

Science Immunology | AMER ASSOC ADVANCEMENT SCIENCE | Published : 2019

Abstract

The role of nonclassical monocytes (NCMs) in health and disease is emerging, but their location and function within tissues remain poorly explored. Imaging of NCMs has been limited by the lack of an established single NCM marker. Here, we characterize the immune checkpoint molecule PD-L1 (CD274) as an unequivocal marker for tracking NCMs in circulation and pinpoint their compartmentalized distribution in tissues by two-photon microscopy. Visualization of PD-L1+ NCMs in relation to bone marrow vasculature reveals that conversion of classical monocytes into NCMs requires contact with endosteal vessels. Furthermore, PD-L1+ NCMs are present in tertiary lymphoid organs (TLOs) under inflammatory c..

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Grants

Awarded by Deutsche Forschungsgemeinschaft


Awarded by European Research Council


Funding Acknowledgements

The present work was supported by the Deutsche Forschungsgemeinschaft grants SFB1123 Z1 (to R.T.A.M.), A1 (to C.W.), A2 (to P.v.H.), A5 (to N.G.), A10 (to J.D. and C.W.), and B9 (to S.S.); MO 3054/1-1 (to S.K.M.); INST409/97-1FUGG and INST409/150-1FUGG (to C.W. and R.T.A.M.); and European Research Council grant ERC_AdGo692511 (to C.W.).