Cholesterol-Dependent Cytolysins: Membrane and Protein Structural Requirements for Pore Formation
Craig J Morton, Marc-Antoine Sani, Michael W Parker, Frances Separovic
CHEMICAL REVIEWS | AMER CHEMICAL SOC | Published : 2019
The cholesterol-dependent cytolysins (CDCs) are a family of bacterial protein toxins specifically targeting eukaryotic cells through the absolute requirement for high concentrations of cholesterol in the target cells' lipid membrane. The soluble monomeric protein secreted by the bacteria oligomerizes on the surface of the target cell, and the complex formed then undergoes a concerted structural transition that results in the creation of a multimeric protein pore. Recognition of the cholesterol-rich membrane by CDCs is a surprisingly subtle process that takes place at the interface between the membrane and surrounding aqueous environment. The structure and composition of the lipid membrane mo..View full abstract
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Awarded by Australian Research Council
This work was supported by an Australian Research Council Discovery Grant DP160101874 to M.W.P. Funding from the Victorian Government Operational Infrastructure Support Scheme to St Vincent's Institute is acknowledged. M.W.P. is a National Health and Medical Research Council of Australia Research Fellow. F.S. acknowledges funding from the Australian Research Council, grant number DP 160100959.