Analysis of Infection Loads in Mycoplasma genitalium Clinical Specimens by Use of a Commercial Diagnostic Test
Gerald L Murray, Jennifer Danielewski, Kaveesha Bodiyabadu, Dorothy A Machalek, Catriona S Bradshaw, Anna-Maria Costa, Josh Birnie, Suzanne M Garland
Journal of Clinical Microbiology | AMER SOC MICROBIOLOGY | Published : 2019
Mycoplasma genitalium is a common sexually transmitted infection with a propensity to acquire resistance to commonly used antimicrobial therapies. Bacterial load has been linked to patient symptoms and the success of treatment. In this study we demonstrate methodology to estimate load from routine diagnostic assays using the ResistancePlus® MG test (SpeeDx Pty Ltd, Australia). The method gave comparable quantitation to an M. genitalium-specific 16S rRNA qPCR (Spearman r=0.94) for the samples analysed (n=499, including urine and swab types as detailed below), and was therefore employed to analyse typical load levels for samples in a diagnostic laboratory (total of 1012 tests). When stratified..View full abstract
Awarded by Department of Industry, Innovation and Science, The Australian Federal Government
This work was supported by an Innovations Connections grant from the Department of Industry, Innovation and Science, The Australian Federal Government (number ICG000220), and the Victorian Medical Research Acceleration Fund, the State Government of Victoria, Australia.