Defining specific allergens for improved component-resolved diagnosis of shrimp allergy in adults
Elecia B Johnston, Sandip D Kamath, Swati P Iyer, Kunal Pratap, Shaymaviswanathan Karnaneedi, Aya C Taki, Roni Nugraha, Patrick M Schaeffer, Jennifer M Rolland, Robyn E O'Hehir, Andreas L Lopata
Molecular Immunology | PERGAMON-ELSEVIER SCIENCE LTD | Published : 2019
Shrimp is one of the predominant causes of food allergy among adults, often presenting with severe reactions. Current in vitro diagnostics are based on quantification of patient specific-IgE (sIgE) to shrimp extract. Tropomyosin is the known major shrimp allergen, but IgE sensitisation to other allergens is poorly characterised. In this study, the binding of IgE to various shrimp allergens, additional to tropomyosin, was investigated using sera from 21 subjects who had clinical reactions to one or more shellfish species. Total shrimp-sIgE was quantified using ImmunoCAP, while allergen-sIgEs were quantified using immunoblotting and mass spectrometry, and immuno-PCR to recombinant shrimp tropo..View full abstract
Awarded by National Health and Medical Research Council Australia
This work is supported by the National Health and Medical Research Council Australia (1086656), the Allergy and Immunology foundation of Australasia (AIFA), and the Queensland Smart Futures Fund (NIRAP, Australia). SK was supported by the National Health and Medical Research Council Australia Research Fellowship (GNT1124143).