Journal article

Estimating neuronal conductance model parameters using dynamic action potential clamp

Y Deerasooriya, G Berecki, D Kaplan, IC Forster, S Halgamuge, S Petrou

Journal of Neuroscience Methods | Elsevier | Published : 2019

Abstract

Parameterization of neuronal membrane conductance models relies on data acquired from current clamp (CC) or voltage clamp (VC) recordings. Although the CC approach provides key information on a neuron’s firing properties, it is often difficult to disentangle the influence of multiple conductances that contribute to the excitation properties of a real neuron. Isolation of a single conductance using pharmacological agents or heterologous expression simplifies analysis but requires extensive VC evaluation to explore the complete state behavior of the channel of interest.

Grants

Awarded by Australian Research Council


Awarded by NHMRC


Awarded by ARC Australian Research Council Centre of Excellence for Integrative Brain Function


Funding Acknowledgements

Y.D. is supported by The University of Melbourne PhD scholarship scheme. This work is also partially funded by Australian Research Council grant DP150103512 to S.H. and NHMRC Research Fellowship grant GNT1005050 and ARC Australian Research Council Centre of Excellence for Integrative Brain Function grant CE140100007 to S.P. S.P. is also a co-founder and equity holder of RogCon, Inc., Miami, USA, and Praxis Precision Medicines, biotechnology companies focused on drug research, discovery and development for ion channel disorders including SCN2A. G.B. was funded by RogCon, Inc.