Journal article

Mice with myocyte deletion of vitamin D receptor have sarcopenia and impaired muscle function

Christian M Girgis, Kuan Minn Cha, Benjamin So, Michael Tsang, Jennifer Chen, Peter J Houweling, Aaron Schindeler, Rebecca Stokes, Michael M Swarbrick, Frances J Evesson, Sandra T Cooper, Jenny E Gunton

JOURNAL OF CACHEXIA SARCOPENIA AND MUSCLE | WILEY | Published : 2019

Abstract

BACKGROUND: It has long been recognized that vitamin D deficiency is associated with muscle weakness and falls. Vitamin D receptor (VDR) is present at very low levels in normal muscle. Whether vitamin D plays a direct role in muscle function is unknown and is a subject of hot debate. Myocyte-specific deletion of VDR would provide a strategy to answer this question. METHODS: Myocyte-specific vitamin D receptor (mVDR) null mice were generated by crossing human skeletal actin-Cre mice with floxed VDR mice. The effects of gene deletion on the muscle phenotype were studied in terms of body tissue composition, muscle tissue histology, and gene expression by real-time PCR. RESULTS: Unlike whole-bod..

View full abstract