Mice with myocyte deletion of vitamin D receptor have sarcopenia and impaired muscle function
Christian M Girgis, Kuan Minn Cha, Benjamin So, Michael Tsang, Jennifer Chen, Peter J Houweling, Aaron Schindeler, Rebecca Stokes, Michael M Swarbrick, Frances J Evesson, Sandra T Cooper, Jenny E Gunton
JOURNAL OF CACHEXIA SARCOPENIA AND MUSCLE | WILEY | Published : 2019
BACKGROUND: It has long been recognized that vitamin D deficiency is associated with muscle weakness and falls. Vitamin D receptor (VDR) is present at very low levels in normal muscle. Whether vitamin D plays a direct role in muscle function is unknown and is a subject of hot debate. Myocyte-specific deletion of VDR would provide a strategy to answer this question. METHODS: Myocyte-specific vitamin D receptor (mVDR) null mice were generated by crossing human skeletal actin-Cre mice with floxed VDR mice. The effects of gene deletion on the muscle phenotype were studied in terms of body tissue composition, muscle tissue histology, and gene expression by real-time PCR. RESULTS: Unlike whole-bod..View full abstract
This work was supported by a Sydney Medical School grant (University of Sydney) and a grant from the Staff Specialist Trust Fund (Westmead Hospital).