Comparative genomic analyses of Mycoplasma synoviae vaccine strain MS-H and its wild-type parent strain 86079/7NS: implications for the identification of virulence factors and applications in diagnosis of M. synoviae
Ling Zhu, Muhammad A Shahid, John Markham, Glenn F Browning, Amir H Noormohammadi, Marc S Marenda
Avian Pathology | Taylor & Francis | Published : 2019
Mycoplasma synoviae is an economically important avian pathogen worldwide, causing respiratory disease, infectious synovitis, airsacculitis and eggshell apex abnormalities in commercial chickens. Despite the widespread use of MS-H as a live attenuated vaccine over the past two decades, the precise molecular basis for loss of virulence in this vaccine is not yet fully understood. To address this, the whole genome sequence of the vaccine parent strain, 86079/7NS, was obtained and compared to that of the MS-H vaccine. Except for the vlhA expressed region, both genomes were nearly identical. Thirty-two single nucleotide polymorphisms (SNPs) were identified in MS-H, including 11 non-synonymous mu..View full abstract
The authors would like to acknowledge the assistance from the staff of Asia-Pacific Centre for Animal Health (APCAH), Faculty of Veterinary and Agricultural Sciences, the University of Melbourne. Funding for this study was provided by APCAH.