Rapamycin Affects Palmitate-Induced Lipotoxicity in Osteoblasts by Modulating Apoptosis and Autophagy
Ahmed Al Saedi, Craig A Goodman, Damian E Myers, Alan Hayes, Gustavo Duque
JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES | OXFORD UNIV PRESS INC | Published : 2020
Bone marrow fat infiltration is one of the hallmarks of aging and osteoporotic bones. Marrow adipocytes produce substantial amounts of palmitic acid (PA). PA is toxic to bone-forming osteoblasts in vitro, affecting their differentiation, function, and survival. Since rapamycin (RAP)-induced inhibition of target of rapamycin complex 1 (mTORC1) activates autophagy and prevents apoptosis, we hypothesized that RAP may preserve osteoblast viability and reduce PA-induced lipotoxicity. Normal human osteoblasts were incubated with RAP in the presence of a lipotoxic concentration of PA or vehicle for 24 and 48 hours. Expression of LC3 protein levels and the phosphorylation of the direct mTORC1 target..View full abstract
Awarded by Australian National Health and Medical Research Council (NHMRC)
This work was supported by a seed grant from the Australian Institute for Musculoskeletal Science (AIMSS) and a grant from the Australian National Health and Medical Research Council (NHMRC) grant NHMRC632767.