Journal article

Heteronuclear NMR spectroscopy of proteins encapsulated in cubic phase lipids

Thomas G Meikle, Ashish Sethi, David W Keizer, Jeffrey J Babon, Frances Separovic, Paul R Gooley, Charlotte E Conn, Shenggen Yao

JOURNAL OF MAGNETIC RESONANCE | ACADEMIC PRESS INC ELSEVIER SCIENCE | Published : 2019

Abstract

Lipidic cubic phases, which form spontaneously via the self-assembly of certain lipids in an aqueous environment, are highly prospective nanomaterials with applications in membrane protein X-ray crystallography and drug delivery. Here we report 1H-15N heteronuclear single/multiple quantum coherence (HSQC, HMQC) spectra of 15N-enriched proteins encapsulated in inverse bicontinuous lipidic cubic phases obtained on a standard commercial high resolution NMR spectrometer at ambient temperature. 15N-enriched proteins encapsulated in this lipidic cubic phase show: (i) no significant changes in tertiary structure, (ii) significantly reduced solvent chemical exchange of backbone amides, which potenti..

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