Postpartum circulating cell-free insulin DNA levels are higher in women with previous gestational diabetes mellitus who develop type 2 diabetes in later life
Martha Lappas, Harry Georgiou, Jane Wilcox, Michael Permezel, Alexis Shub, Cody-Lee Maynard, Mugdha Joglekar, Anandwardhan HARDIKAR
Journal of Diabetes Research | Hindawi Publishing Corporation | Published : 2019
Background. Women with previous gestational diabetes mellitus (GDM) have evidence of postpartum β-cell dysfunction, which increases their risk of developing type 2 diabetes (T2DM) later in life. Elevated levels of circulating cell-free preproinsulin (INS) DNA correlate with dying β-cells in both mice and humans. The aim of this study was to determine if cell-free circulating INS DNA levels are higher in women with previous GDM who develop T2DM. Methods. We used droplet digital (dd) PCR to measure the levels of cell-free circulating methylated and unmethylated INS DNA in plasma from 97 women with normal glucose tolerance (NGT), 12 weeks following an index GDM pregnancy. Women were assessed ..View full abstract
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Associate Professor Martha Lappas is supported by a Research Fellowship from the Department of Obstetrics and Gynaecology (University of Melbourne) and a Faculty Fellowship from the University of Melbourne. The work described in this manuscript was funded by the Norman Beischer Medical Research Foundation (ML, AS, and HMG) and Diabetes Australia (ML). AAH, MVJ, and CM are supported through a fellowship from the Juvenile Diabetes Research Foundation Australia (JDRF), the JDRF International, and the Sydney Medical Foundation and Sydney University Summer Student program. Infrastructure/research support to AAH through grants from the JDRF Australia, the Rebecca L. Cooper Foundation, and the NHMRC CTC, University of Sydney, are acknowledged. Debra Jinks, Connie CJ Louizos, Amy Bohren, and Sonya Ristevski are thanked for their assistance with the recruitment of the women. Raghu Mirmira and Sarah Tersey, Indiana University, are thanked for sharing their cfDNA protocols and plasmids.