Secondary Structural Changes in Proteins as a Result of Electroadsorption at Aqueous-Organogel Interfaces
Samuel G Booth, Bren Mark B Felisilda, Eva Alvarez de Eulate, Ove JR Gustafsson, Mahreen Arooj, Ricardo L Mancera, Robert AW Dryfe, Mark J Hackett, Damien WM Arrigan
Langmuir | AMER CHEMICAL SOC | Published : 2019
The electroadsorption of proteins at aqueous-organic interfaces offers the possibility to examine protein structural rearrangements upon interaction with lipophilic phases, without modifying the bulk protein or relying on a solid support. The aqueous-organic interface has already provided a simple means of electrochemical protein detection, often involving adsorption and ion complexation; however, little is yet known about the protein structure at these electrified interfaces. This work focuses on the interaction between proteins and an electrified aqueous-organic interface via controlled protein electroadsorption. Four proteins known to be electroactive at such interfaces were studied: lyso..View full abstract
S.G.B. gratefully acknowledges the Curtin Institute for Functional Molecules and Interfaces for provision of funding to support his research visit to Curtin University. B.M.B.F. acknowledges Curtin University for the award of a Curtin International Postgraduate Research Scholarship.