Journal article

Chronically stimulated human MAIT cells are unexpectedly potent IL-13 producers

Jason Kelly, Yosuke Minoda, Tobias Meredith, Garth Cameron, Marie-Sophie Philipp, Daniel G Pellicci, Alexandra J Corbett, Christian Kurts, Daniel HD Gray, Dale Godfrey, George Kannourakis, Stuart P Berzins

Immunology and Cell Biology | WILEY | Published : 2019

Abstract

Mucosal-associated invariant T (MAIT) cells are unconventional T cells that recognize antigens derived from riboflavin biosynthesis. In addition to anti-microbial functions, human MAIT cells are associated with cancers, autoimmunity, allergies and inflammatory disorders, although their role is poorly understood. Activated MAIT cells are well known for their rapid release of Th1 and Th17 cytokines, but we have discovered that chronic stimulation can also lead to potent interleukin (IL)-13 expression. We used RNA-seq and qRT-PCR to demonstrate high expression of the IL-13 gene in chronically stimulated MAIT cells, and directly identify IL-13 using intracellular flow cytometry and multiplex bea..

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Grants

Awarded by National Health and Medical Research Council of Australia (NHMRC)


Awarded by Australian Research Council (ARC)


Awarded by CASS Foundation


Awarded by Deutsche Forschungsgemeinschaft


Awarded by ARC Future Fellowship


Awarded by Australian NHMRC Senior Research Fellowship


Awarded by NHMRC Senior Principal Research Fellowship


Funding Acknowledgements

This research was supported by the National Health and Medical Research Council of Australia (NHMRC) (1113293, 1140126, 1145888 and 1121325), Australian Research Council (ARC) (CE140100011), CASS Foundation (8510) and FECRI. JK was supported by a PhD scholarship co-funded by Lung Foundation Australia and an Australian Government Research Training Program (RTP) Fee-Offset Scholarship through Federation University Australia. MP was supported by the Deutsche Forschungsgemeinschaft-funded graduate school GRK2168 and the The Bonn and Melbourne Research and Graduate School (Bo&MeRanG) Program. DP is supported by a CSL Centenary Fellowship. AC is an inventor on patents describing MR1-tetramers and is supported by an ARC Future Fellowship FT160100083. DHDG was supported by Australian NHMRC Senior Research Fellowship (1158024). DG is supported by an NHMRC Senior Principal Research Fellowship (1117766). SB is supported by a Dorevitch Cancer Research Fellowship. Mr Bruce Stewart performed the surgeries that provided colon tissue. The authors acknowledge The Department of Histology at Australian Clinical Labs, St John of God Hospital, Ballarat, for processing of paraffin embedded tissue and Professor James McCluskey for provision of MR1 reagents. The authors acknowledge the generous contributions of donors and clinicians who helped to provide human tissue samples.