Journal article

Junction-based lamellipodia drive endothelial cell rearrangements in vivo via a VE-cadherin-F-actin based oscillatory cell-cell interaction

Ilkka Paatero, Loic Sauteur, Minkyoung Lee, Anne K Lagendijk, Daniel Heutschi, Cora Wiesner, Camilo Guzman, Dimitri Bieli, Benjamin M Hogan, Markus Affolter, Heinz-Georg Belting

NATURE COMMUNICATIONS | NATURE PUBLISHING GROUP | Published : 2018

Abstract

Angiogenesis and vascular remodeling are driven by extensive endothelial cell movements. Here, we present in vivo evidence that endothelial cell movements are associated with oscillating lamellipodia-like structures, which emerge from cell junctions in the direction of cell movements. High-resolution time-lapse imaging of these junction-based lamellipodia (JBL) shows dynamic and distinct deployment of junctional proteins, such as F-actin, VE-cadherin and ZO1, during JBL oscillations. Upon initiation, F-actin and VE-cadherin are broadly distributed within JBL, whereas ZO1 remains at cell junctions. Subsequently, a new junction is formed at the front of the JBL, which then merges with the prox..

View full abstract

University of Melbourne Researchers

Grants

Funding Acknowledgements

We thank Kumuthini Kulendra for fish care and the Imaging Core Facility of the Biozentrum (University of Basel) for microscopy support. We thank Johanna Ivaska for support and acknowledge Zebrafish Core Facility (Turku Centre for Biotechnology, University of Turku and Abo Akademi University). This work has been supported by the Kantons Basel-Stadt and Basel-Land and by a grant from the Swiss National Science Foundation to M.A. I.P. was supported by a post-doctoral fellowship from the Finnish Cultural Foundation and Foundations' Post-Doc Pool. M.L., C.W., and L.S. were supported by a Fellowship of Excellence, Biozentrum, University of Basel.