Sox18 Genetically Interacts With VegfC to Regulate Lymphangiogenesis in Zebrafish
Solei Cermenati, Silvia Moleri, Christine Neyt, Erica Bresciani, Silvia Carra, Daniela R Grassini, Alice Omini, Michela Goi, Franco Cotelli, Mathias Francois, Benjamin M Hogan, Monica Beltrame
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY | LIPPINCOTT WILLIAMS & WILKINS | Published : 2013
OBJECTIVE: Lymphangiogenesis is regulated by transcription factors and by growth factor pathways, but their interplay has not been extensively studied so far. We addressed this issue in zebrafish. APPROACH AND RESULTS: Mutations in the transcription factor-coding gene SOX18 and in VEGFR3 cause lymphedema, and the VEGFR3/Flt4 ligand VEGFC plays an evolutionarily conserved role in lymphangiogenesis. Here, we report a strong genetic interaction between Sox18 and VegfC in the early phases of lymphatic development in zebrafish. Knockdown of sox18 selectively impaired lymphatic sprouting from the cardinal vein and resulted in defective lymphatic thoracic duct formation. Sox18 and the related prote..View full abstract
Awarded by Regione Lombardia
Awarded by Fondazione Cariplo
Awarded by Australian Research Council Future Fellowship
We acknowledge financial support by Regione Lombardia (grant SAL-01 to M. Beltrame) and by Fondazione Cariplo (grant 2011-0555 to M. Beltrame). B.M. Hogan was supported by an Australian Research Council Future Fellowship (FT100100165).