Journal article

Genome Mining Reveals Endopyrroles from a Nonribosomal Peptide Assembly Line Triggered in Fungal-Bacterial Symbiosis

Sarah P Niehs, Benjamin Dose, Kirstin Scherlach, Sacha J Pidot, Timothy P Stinear, Christian Hertweck

ACS Chemical Biology | AMER CHEMICAL SOC | Published : 2019

Abstract

The bacterial endosymbiont (Burkholderia rhizoxinica) of the rice seedling blight fungus (Rhizopus microsporus) harbors a large number of cryptic biosynthesis gene clusters. Genome mining and sequence similarity networks based on an encoded nonribosomal peptide assembly line and the associated pyrrole-forming enzymes in the symbiont indicated that the encoded metabolites are unique among a large number of tentative pyrrole natural products in diverse and unrelated bacterial phyla. By performing comparative metabolic profiling using a mutant generated with an improved pheS Burkholderia counterselection marker, we found that the symbionts' biosynthetic pathway is mainly activated under salt st..

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Grants

Awarded by DFG


Funding Acknowledgements

We thank H.-M. Dahse and C. Weigel for bioactivity testing, A. Perner for MS/MS, and H. Heinecke for NMR measurements. We also gratefully acknowledge S. Richter for assistance in compound isolation and Dr. K. Dunbar for helpful discussions. This work was financially supported by the DFG (SFB 1127, ChemBioSys, and Leibniz Award to C.H.).