SopF, a phosphoinositide binding effector, promotes the stability of the nascent Salmonella-containing vacuole

N Lau; AL Haeberle; BJ O’Keeffe; EA Latomanski; J Celli; HJ Newton; LA Knodler

Journal article

SopF, a phosphoinositide binding effector, promotes the stability of the nascent Salmonella-containing vacuole

N Lau, AL Haeberle, BJ O’Keeffe, EA Latomanski, J Celli, HJ Newton, LA Knodler

Plos Pathogens | PUBLIC LIBRARY SCIENCE | Published : 2019

DOI: 10.1371/journal.ppat.1007959

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Abstract

The enteric bacterial pathogen Salmonella enterica serovar Typhimurium (S. Typhimurium), utilizes two type III secretion systems (T3SSs) to invade host cells, survive and replicate intracellularly. T3SS1 and its dedicated effector proteins are required for bacterial entry into non-phagocytic cells and establishment and trafficking of the nascent Salmonella-containing vacuole (SCV). Here we identify the first T3SS1 effector required to maintain the integrity of the nascent SCV as SopF. SopF associates with host cell membranes, either when translocated by bacteria or ectopically expressed. Recombinant SopF binds to multiple phosphoinositides in protein-lipid overlays, suggesting that it target..

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University of Melbourne Researchers

Hayley Newton Author

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Grants

Awarded by University of Texas Southwestern Medical Center

Funding Acknowledgements

This study was funded by a grant from the National Institutes of Health (NIH)/National Institute of Allergy and Infectious Disease (NIAID; https://www.niaid.nih.gov) R21AI130645 to LAK; a grant from the National Health and Medical Research Council (NHMRC; https://nhmrc.gov.au/) APP1120344 to HJN; and a grant from the Australian Research Council (ARC; https://www.arc. gov. au/) DP180101298 to HJN. NL was partially supported by a stipend from the NHMRC Program in Cellular Microbiology (http://purl.org/au-research/grants/nhmrc/1092262).LAK holds an Investigators in the Pathogenesis of Infectious Disease Award from the Burroughs Wellcome Fund. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.