Malaria surveillance from both ends: concurrent detection of Plasmodium falciparum in saliva and excreta harvested from Anopheles mosquitoes
Ana L Ramirez, Andrew F van den Hurk, Ian M Mackay, Annie SP Yang, Glen R Hewitson, Jamie L McMahon, Justin A Boddey, Scott A Ritchie, Sara M Erickson
Parasites and Vectors | BMC | Published : 2019
BACKGROUND: Malaria is the most important vector-borne disease in the world. Epidemiological and ecological studies of malaria traditionally utilize detection of Plasmodium sporozoites in whole mosquitoes or salivary glands by microscopy or serological or molecular assays. However, these methods are labor-intensive, and can over- or underestimate mosquito transmission potential. To overcome these limitations, alternative sample types have been evaluated for the study of malaria. It was recently shown that Plasmodium could be detected in saliva expectorated on honey-soaked cards by Anopheles stephensi, providing a better estimate of transmission risk. We evaluated whether excretion of Plasmod..View full abstract
Awarded by NHMRC
Awarded by National Health and Medical Research Council
This work was supported by a Queensland Government funded Australian Institute of Tropical Health and Medicine Capacity Building Grant. Insectary support was provided by the Walter and Eliza Hall Institute, NHMRC Project Grant 1049811 and a Victorian State Government Operational Infrastructure Support and Australian Government NHMRC IRIISS. ALR was supported by a doctoral scholarship granted by the Australian Institute of Tropical Health and Medicine, James Cook University QLD, Australia. JAB and SAR were supported, in part, by National Health and Medical Research Council research fellowships 1123727 and 1044698, respectively. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.