Journal article

The Measurement of Donor-Specific Cell-Free DNA Identifies Recipients With Biopsy-Proven Acute Rejection Requiring Treatment After Liver Transplantation

Su Kah Goh, Hongdo Do, Adam Testro, Julie Pavlovic, Angela Vago, Julie Lokan, Robert M Jones, Christopher Christophi, Alexander Dobrovi, Vijayaragavan Muralidhara

Transplantation Direct | LIPPINCOTT WILLIAMS & WILKINS | Published : 2019

Abstract

Background: Assessment of donor-specific cell-free DNA (dscfDNA) in the recipient is emerging as a noninvasive biomarker of organ rejection after transplantation. We previously developed a digital polymerase chain reaction (PCR)-based approach that readily measures dscfDNA within clinically relevant turnaround times. Using this approach, we characterized the dynamics and evaluated the clinical utility of dscfDNA after liver transplantation (LT). Methods: Deletion/insertion polymorphisms were used to distinguish donor-specific DNA from recipient-specific DNA. Posttransplant dscfDNA was measured in the plasma of the recipients. In the longitudinal cohort, dscfDNA was serially measured at days ..

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Grants

Funding Acknowledgements

This study was supported by Austin Medical Research Foundation, Austin Health; Research Scholarship, Australian and New Zealand Hepatic, Pancreatic & Biliary Association; Foundation of Surgery, Royal Australasian College of Surgeons; Victorian Liver Transplantation Unit, Austin Health; Department of Surgery, The University of Melbourne, Austin Health; and Operational Infrastructure Support Program Funding of the Victorian State Government.