Differential susceptibility of human neural progenitors and neurons to ischaemic injury
Ye Liu, Anna E Michalska, Mirella Dottori, Emma Eaton, Jo-Maree Courtney, Ana Antonic, David W Howells
BRAIN RESEARCH BULLETIN | PERGAMON-ELSEVIER SCIENCE LTD | Published : 2020
BACKGROUND: Neuroprotection for stroke has shown great promise but has had little translational success. Developing drugs for humans logically requires human tissue evaluation. Human embryonic stem cell (hESC)-derived neuronal cultures at different developmental stages were subject to oxygen glucose deprivation (OGD) to determine how developing maturity altered response to ischemic injury. METHODS: H9 hESCs were induced by Noggin to generate neural progenitors (NPs) and highly arbourised structurally complex neurons. They were both subjected to OGD or OGD with reoxygenation (OGD-R) for 1-6 h.Outcome was assessed by measures of cell death, survival and morphology. RESULTS: NPs did not die aft..View full abstract
Awarded by Australian National Health and Medical Research Council (NHMRC)
This work was supported by the Australian National Health and Medical Research Council (NHMRC), grant number: 1013621(Improving Stroke Outcomes: Attenuating Progression and Recurrence) and 1037863(Biomarkers for assessing the effectiveness of hypothermia as a therapy in ischemic stroke patients).