Conference Proceedings

Differential susceptibility of human neural progenitors and neurons to ischaemic injury

Ye Liu, Anna E Michalska, Mirella Dottori, Emma Eaton, Jo-Maree Courtney, Ana Antonic, David W Howells

BRAIN RESEARCH BULLETIN | PERGAMON-ELSEVIER SCIENCE LTD | Published : 2020

Abstract

BACKGROUND: Neuroprotection for stroke has shown great promise but has had little translational success. Developing drugs for humans logically requires human tissue evaluation. Human embryonic stem cell (hESC)-derived neuronal cultures at different developmental stages were subject to oxygen glucose deprivation (OGD) to determine how developing maturity altered response to ischemic injury. METHODS: H9 hESCs were induced by Noggin to generate neural progenitors (NPs) and highly arbourised structurally complex neurons. They were both subjected to OGD or OGD with reoxygenation (OGD-R) for 1-6 h.Outcome was assessed by measures of cell death, survival and morphology. RESULTS: NPs did not die aft..

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Grants

Awarded by Australian National Health and Medical Research Council (NHMRC)


Funding Acknowledgements

This work was supported by the Australian National Health and Medical Research Council (NHMRC), grant number: 1013621(Improving Stroke Outcomes: Attenuating Progression and Recurrence) and 1037863(Biomarkers for assessing the effectiveness of hypothermia as a therapy in ischemic stroke patients).