Journal article

The structure of the influenza A virus genome

Bernadeta Dadonaite, Brad Gilbertson, Michael L Knight, Sanja Trifkovic, Steven Rockman, Alain Laederach, Lorena E Brown, Ervin Fodor, David LV Bauer

NATURE MICROBIOLOGY | NATURE PUBLISHING GROUP | Published : 2019

Abstract

Influenza A viruses (IAVs) constitute a major threat to human health. The IAV genome consists of eight single-stranded viral RNA segments contained in separate viral ribonucleoprotein (vRNP) complexes that are packaged together into a single virus particle. The structure of viral RNA is believed to play a role in assembling the different vRNPs into budding virions1-8 and in directing reassortment between IAVs9. Reassortment between established human IAVs and IAVs harboured in the animal reservoir can lead to the emergence of pandemic influenza strains to which there is little pre-existing immunity in the human population10,11. While previous studies have revealed the overall organization of ..

View full abstract

Grants

Awarded by Wellcome Trust


Awarded by UK Biotechnology and Biological Sciences Research Council


Awarded by National Institutes of Health


Awarded by National Health and Medical Research Council of Australia


Awarded by UK Medical Research Council programme


Awarded by NATIONAL HUMAN GENOME RESEARCH INSTITUTE


Funding Acknowledgements

We thank G.G. Brownlee and J. Kenyon for helpful discussions, J. Kenyon, J.G. Aw, and Y. Wan for sharing protocols, J. Robertson for making the 1M7 reagent, J. Sharps for technical assistance and St Jude Children's Research Hospital for providing the pHW2000 plasmid. This work was supported by a Wellcome Trust studentship (no. 105399/Z/14/Z to B.D.), a UK Biotechnology and Biological Sciences Research Council studentship (no. BB/M011224/1 to M.L.K.), grants from the National Institutes of Health (nos. HL111527, GM101237 and HG008133 to A.L.), a National Health and Medical Research Council of Australia programme grant (no. ID1071916 to L.E.B.), UK Medical Research Council programme grants (nos. MR/K000241/1 and MR/R009945/1 to E.F.) and a Sir Edward Penley Abraham Cephalosporin Junior Research Fellowship to D.L.V.B.