Journal article
Bmp and Nodal independently regulate lefty1 expression to maintain unilateral Nodal activity during left-right axis specification in zebrafish
KA Smith, E Noël, I Thurlings, H Rehmann, S Chocron, J Bakkers
Plos Genetics | PUBLIC LIBRARY SCIENCE | Published : 2011
Open access
Abstract
In vertebrates, left-right (LR) axis specification is determined by a ciliated structure in the posterior region of the embryo. Fluid flow in this ciliated structure is responsible for the induction of unilateral left-sided Nodal activity in the lateral plate mesoderm, which in turn regulates organ laterality. Bmp signalling activity has been implied in repressing Nodal expression on the right side, however its mechanism of action has been controversial. In a forward genetic screen for mutations that affect LR patterning, we identified the zebrafish linkspoot (lin) mutant, characterized by cardiac laterality and mild dorsoventral patterning defects. Mapping of the lin mutation revealed an in..
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Funding Acknowledgements
KAS was supported by a Concordia fellowship from Stichting Vrienden van het Hubrecht (http://www.hubrecht.eu/information/foundation.html). Research in JB's laboratory was supported by the Netherlands Organisation for Scientific Research (NWO/ALW) grant 864.08.009 (http://www.nwo.nl/nwohome.nsf/pages/SPPD_7AK3L2_Eng). The bmpr1absa0028 zebrafish mutant was generated as part of the ZF-MODELS Integrated Project in the 6th Framework Programme (Contract No. LSHG-CT-2003-503496) funded by the European Commission (http://cordis.europa.eu/fp6/dc/index.cfm?fuseaction=UserSite.FP6HomePage). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.