Journal article

RPW8/HR repeats control NLR activation in Arabidopsis thaliana

Cristina A Barragan, Rui Wu, Sang-Tae Kim, Wanyan Xi, Anette Habring, Joerg Hagmann, Anna-Lena Van de Weyer, Maricris Zaidem, William Wing, Ho Ho, George Wang, Ilja Bezrukov, Detlef Weigel, Eunyoung Chae

PLOS Genetics | PUBLIC LIBRARY SCIENCE | Published : 2019

Abstract

In many plant species, conflicts between divergent elements of the immune system, especially nucleotide-binding oligomerization domain-like receptors (NLR), can lead to hybrid necrosis. Here, we report deleterious allele-specific interactions between an NLR and a non-NLR gene cluster, resulting in not one, but multiple hybrid necrosis cases in Arabidopsis thaliana. The NLR cluster is RESISTANCE TO PERONOSPORA PARASITICA 7 (RPP7), which can confer strain-specific resistance to oomycetes. The non-NLR cluster is RESISTANCE TO POWDERY MILDEW 8 (RPW8) / HOMOLOG OF RPW8 (HR), which can confer broad-spectrum resistance to both fungi and oomycetes. RPW8/HR proteins contain at the N-terminus a potent..

View full abstract

University of Melbourne Researchers

Grants

Awarded by Marie Curie postdoctoral fellowship


Awarded by National University of Singapore


Awarded by ERC


Awarded by Deutsche Forschungsgemeinschaft through the Collaborative Research Center


Funding Acknowledgements

This study was supported by a Marie Curie postdoctoral fellowship (2014-655295, https://ec.europa.eu/research/mariecurieactions/actions/individual-fellowships_en) to RW, the Academic Research Fund from the National University of Singapore (R-154-000-B33-114, https://www.nrf.gov.sg/) to EC, ERC Advanced Grant IMMUNEMESIS (340602, https://erc.europa.eu/funding/advanced-grants), the Deutsche Forschungsgemeinschaft through the Collaborative Research Center (CRC1101, https://www.dfg.de/en/research_funding/programmes/coordinated_programmes/collaborative_research_centres/), and The Max Planck Society (https://www.mpg.de/en) to DW. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.