Non-invasive screening of a microRNA-based dysregulation signature in oral cancer and oral potentially malignant disorders
T Yap, C Seers, K Koo, L Cheng, LJ Vella, AF Hill, E Reynolds, A Nastri, N Cirillo, M McCullough
ORAL ONCOLOGY | ELSEVIER | Published : 2019
INTRODUCTION: We have previously shown that oral swirls are a robust source of microRNA protected by extracellular vesicles, potentially useful to detect oral squamous cell carcinoma (OSCC)-associated molecular aberration. OBJECTIVES: To study a developed dysregulation score and risk classification algorithm based upon a panel of OSCC-associated microRNA in oral swirls from individuals with OSCC and oral potentially malignant disorders (OPMDs). MATERIALS AND METHODS: An OSCC-associated panel of 5 microRNAs (miR-24; miR-21; miR-99a; let-7c; miR-100;) was quantified by qPCR in 190 individuals with and without mucosal abnormalities, including OSCC (n = 53) and OPMDs (n = 74). Each sample was an..View full abstract
The authors would like to acknowledge Professor Mats Jontell and Mr Anders Gripp, Institute of Odontology, University of Gothenburg, for the utility and support of MForm as well as Dr Cameron Patrick, School of Mathematics and Statistics, University of Melbourne. This research would not be possible without the generous support of the Australian Dental Research Foundation, The Australia and New Zealand Head and Neck Cancer Society Research Fund and the National Health and Research Council (NHMRC).