Cellular Targeting of Bispecific Antibody-Functionalized Poly(ethylene glycol) Capsules: Do Shape and Size Matter?
Danzi Song, Jiwei Cui, Yi Ju, Matthew Faria, Huanli Sun, Christopher B Howard, Kristofer J Thurecht, Frank Caruso
ACS Appl Mater Interfaces | American Chemical Society | Published : 2019
In the present study, a capsule system that consists of a stealth carrier based on poly(ethylene glycol) (PEG) and functionalized with bispecific antibodies (BsAbs) is introduced to examine the influence of the capsule shape and size on cellular targeting. Hollow spherical and rod-shaped PEG capsules with tunable aspect ratios (ARs) of 1, 7, and 18 were synthesized and subsequently functionalized with BsAbs that exhibit dual specificities to PEG and epidermal growth factor receptor (EGFR). Dosimetry (variation between the concentrations of capsules present and capsules that reach the cell surface) was controlled through "dynamic" incubation (i.e., continuously mixing the incubation medium). ..View full abstract
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Awarded by Australian Research Council (ARC)
This research was conducted and funded by the Australian Research Council (ARC) Centre of Excellence in Convergent Bio-Nano Science and Technology (project no. CE140100036). F.C. acknowledges the award of National Health and Medical Research Council (NHMRC) Senior Principal Research Fellowship (GNT1135806) and K.J.T. acknowledges the award of an NHMRC Career Development Fellowship (APP1148582). This work was performed in part at the Materials Characterisation and Fabrication Platform (MCFP) at The University of Melbourne, AMREP Flow Cytometry Core Facility at Burnet Institute and both the Victorian and Queensland Nodes of the Australian National Fabrication Facility (ANFF).