Anti-Diabetic, Anti-Inflammatory, and Anti-Oxidant Effects of Naringenin in an In Vitro Human Model and an In Vivo Murine Model of Gestational Diabetes Mellitus
Caitlyn Nguyen-Ngo, Jane C Willcox, Martha Lappas
Molecular Nutrition & Food Research | WILEY | Published : 2019
SCOPE: Gestational diabetes mellitus (GDM), which affects up to 20% of pregnant women, is associated with maternal peripheral insulin resistance, low-grade inflammation, and oxidative stress. The flavonoid naringenin has potent anti-diabetic, anti-inflammatory, and anti-oxidative properties; however, its effects in GDM remain unknown. The study aimed to determine the effects of naringenin on glucose metabolism, inflammation, and oxidative stress associated with GDM both in vitro and in vivo. METHODS AND RESULTS: In vitro, human tissue samples obtained at term elective Caesarean section are stimulated with tumour necrosis factor alpha (TNF) to develop a GDM-like environment. Naringenin treatm..View full abstract
M.L. conceived and designed the study, and critically reviewed the manuscript. C.N.-N. performed experiments, analyzed the data, and wrote the manuscript. J.C.W. critically reviewed the study and the manuscript. Associate Professor Martha Lappas is supported by a Research Fellowship from the Department of Obstetrics and Gynaecology (University of Melbourne) and a Faculty Fellowship from the University of Melbourne. C.N.-N. was supported by an Australian Government Research Training Program (RTP) scholarship. J.C.W. was supported by a La Trobe University post-doctoral fellowship. This study was supported by grants from the Norman Beischer Medical Research Foundation, Diabetes Australia, Austin Medical Research Foundation, Rebecca L. Cooper Foundation, the University of Melbourne, and the Mercy Research Foundation. The authors acknowledge Dr. Ratana Lim and Dr. Stella Liong for their assistance with the in vivo mouse study; Clinical Research Midwives Gabrielle Pell, Genevieve Christophers, and Rachel Murdoch for sample collection; the Obstetrics and Midwifery staff of the Mercy Hospital for Women for their co-operation; the women who generously donated their tissue samples to the study; and the Austin BioResource Facility staff for the breeding and care of the housing and maintenance of the GDM mice.