Journal article

CD31 delays phagocyte membrane repolarization to promote efficient binding of apoptotic cells

Elizabeth F Vernon-Wilson, Frederic Aurad, Lijun Tian, Iain CM Rowe, Michael J Shipston, John Savill, Simon B Brown

JOURNAL OF LEUKOCYTE BIOLOGY | FEDERATION AMER SOC EXP BIOL | Published : 2007

Abstract

Homophilic ligation of CD31, a member of the Ig superfamily of adhesion receptors, promotes macrophage clearance of apoptotic leukocytes by a mechanism hitherto not described. In studying CD31-dependent regulation of beta1-integrin binding of fibronectin-coated Latex beads, we discovered a role for the voltage-gated potassium channel ether-à-go-go-related gene (ERG) as a downstream effector of CD31 signaling. ERG was identified by tandem mass spectrometry as a 140-kDa protein, which was selectively modified with biotin following the targeted delivery of a biotin-transfer reagent to CD31 using Fab fragments of an anti-CD31 mAb. Similar results were obtained with macrophages but not K562 cells..

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