Journal article

The death of human platelets during incubation in citrated plasma involves shedding of CD42b and aggregation of dead platelets

PS Hartley, J Savill, SB Brown

THROMBOSIS AND HAEMOSTASIS | SCHATTAUER GMBH-VERLAG MEDIZIN NATURWISSENSCHAFTEN | Published : 2006

Abstract

The ability to readily identify dead platelets is invaluable to studies examining the means of their death, factors affecting their lifespan and their means of clearance by phagocytes. The aim of the present work was to develop a vital staining procedure for the rapid and objective discrimination of live from dead platelets that accrued in citrated platelet rich plasma (cPRP) incubated at 37 degrees C for several days. By transmission electron microscopy it was noted that platelet death was morphologically similar to necrosis and associated with aggregate formation. The vital dyes calcein-AM and FM 4-64 were found to robustly report the death of platelets and indicated that the aggregates wh..

View full abstract