Real-time demultiplexing Nanopore barcoded sequencing data with npBarcode
Hoang Nguyen Son, Tania PS Duarte, Lachlan JM Coin, Duc Cao Minh
BIOINFORMATICS | OXFORD UNIV PRESS | Published : 2017
Motivation: The recent introduction of a barcoding protocol for Oxford Nanopore sequencing has increased the versatility of the technology. Several bioinformatics tools have been developed to demultiplex barcoded reads, but none of them supports streaming analysis. This limits the use of multiplexed sequencing in real-time applications, which is one of the main advantages of the technology. Results: We introduced npBarcode, an open source and cross-platform tool for barcode demultiplexing in streaming fashion that can be used to pipe data to further real-time analyses. The tool also provides a friendly graphical user interface by integrating the module into npReader, making possible to monit..View full abstract
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Awarded by National Health and Medical Research Council
The research is supported by funding from the National Health and Medical Research Council (APP1052303). Lachlan Coin is an NHMRC career development fellow (APP1130084).